Publications
Home Page
Lab Phone 4-5015
Site Administrator
copyright 2003-2009 dhaudi
Molecular Biology (DNA, RNA, Plasmid isolation and manipulation)
  • Alginate Bead Culture
  • Alginate Culture (Anne)
  • Alginate Culture (Kuo)
  • Alk-Phos Assay 96-well kinetic
  • Alk-Phos Direct Labeling
  • AminoLink Gel Coupling
  • Biotinylation of Proteins
  • cDNA Synthesis with ThermoScript (Gibco)
  • Cell line characterization on microcarriers
  • Colony Screening
  • Copy Number Calculations
  • Degenerate primer design
  • DEPC Water
  • DNA Cleanup on Chromaspin columns
  • DNA Precipitation using TCA
  • DNA Sequencing AmpliCycle Kit
  • DNA Sequencing using Dye termination for ABI-377 improved
  • Electroporation of E.Coli
  • Electroporation of LNCaP Cells
  • Electroporation of in vitro Cells
  • ExpressHyb
  • ExpressHyb protocol
  • Filling in overhangs to get blunt ends
  • Genomic DNA Isolation
  • ImmunoHistoChemistry of RAGS
  • KinaseMax™ Oligolabeling
  • LB medium
  • Mercaptoethanol Safety
  • MESA buffer
  • Mini-Prep 10-minute from APS
  • Mini-Prep Alk-Lysis mini-prep large
  • Mini-Prep Alk-Lysis mini-prep 96-well size
  • Ni-NTA Agarose Purification of 6-His Proteins
  • Northern Hybridizations
  • Northern Blotting setup
  • Nuclease Protection Assay (Multi-NPA)
  • Retrovirus Production in GP2-293 cells
  • Random Prime Labelling (Stratagene)
  • Recombinant Protein from Pichia
  • RNA from Agarose (Modified Li Trick)
  • RNA from cartilage (Ruth's protocol)
  • RNA Later (Grayson's Protocol)
  • Degenerate PCR primer design
  • RNeasy with DNAse Treatment
  • SB buffer (TBE alternative)
  • Southern Blotting Setup
  • Southrn Hybridization per APS
  • SSC, 20x
  • Sulfo-Link Coupling of peptides to columns
  • Sulfate Incorporation by Alcian Blue Precipitation
  • SuperFect Transfections
  • Transformation of Plasmid into XL1-Blue Competent Cells
  • TB Medium
  • TBE 10x
  • TCA Precipitation of Proteins from Yeast Media
  • Viral Infection of Stem Cells with mmlv-based virus
  • Western Blot Stripping for Re-use
  • Yeast Media
    • Cell Biology (Cell Culture, isolation and manipulation)
  • Gel Purify Genomic DNA
  • Genomic DNA Isolation
  • Chondrocyte Isolation from Human Samples
  • Chondrogenesis Media
  • Chondrogenesis Media for Alginate Cultures
  • Stem Cells (pleuripotential cells anyways) from Human Bone Marrow
  • Cartilage Explant Injury Protocol
  • Lipofection of SW1353 Chondrosarcoma
  • QuickHyb Protocol
  • RapicHyb Protocol
  • RNA from Cartilage using Cesium Chloride cushion
  • Southern Blot Setup
  • Transfection of Cos-7 cells
  • Western Blotting Buffers used at Genzyme
  • Western Blotting using 3C7 anti-TGF-Beta
    • Protein isolation, purification, analysis, immunochemistry
  • 45-Calcium Binding Assay
  • Agarose Proteins Gels for Rabbit Immunization
  • Ammonium Persulfate 10%
  • Ampicillin Stock Solution
  • Antibiotics [] in Bacterial Plates
  • Antigen Retrieval Protocol for Immunohistochemistry
  • Bacterial Lysis for Protein Preps
  • Biotinylation of Link Protein and Cartilage Matrix Protein
  • Bouin's Fixative
  • Calcium Chloride Competent Bacteria
  • Calcium Chloride Bacterial Transformations
  • Carnoy's Fixative and its use
  • Cell Fractionation from Cultures
  • Cesium Chloride Table
  • Cleaning and Coating Slides
  • Cloning by Expression in Bacteria
  • Cloning from PCR products
  • Cyanogen-Bromide-Sepharose Coupling Buffer
  • Collagen Fibril Extraction and Purification
  • Coomassie Blue Stain for Protein Gels
  • Coupling of Proteins to Cyanogen-Bromide-Sepharose
  • CPC Precipitation of PolyAnions
  • Cesium Chloride Gradient for Cartilage (A1D1 etc)
  • DEAE-Dextran Transfection
  • Dehhardt's Solution
  • Digestion of Proteins
  • DNA Isolation from Agarose using Agarase
  • DNA Isolation from Agarose
  • DNA Isolation from Agarose using Prep-A-Gene (BioRad)
  • DNA Mini-Prep by CTAB method
  • E-6 Color Slide Development
  • E.Coli with P3 Plasmid use and theory
  • ECM Coating
  • EDTA 0.5M Stock Solution
  • Electroporation competent E.Coli
  • Electroporation of E.Coli
  • ELISA Procedure from Eva Engvall
  • EM Negative Staining
  • Extraction and Purification of Collagen Fibrils
  • Factor Xa Cleavage of Pecombinant Proteins
  • Fibrillogenesis Assay
  • Fixation Chart
  • Formamide De-Ionization
  • Freezing Cells for Tissue-Culture
  • Hemacytometer Use
  • Hybridization Mix for Southerns
  • Hybridoma Fusion to make Monoclonal Antibodies
  • Immunocytochemistry
  • ImmunoFluorescence
  • ImmunoFluorescence (more)
  • Immunoperixidase Staining
  • Immuno-Precipitations from Cells
  • Immuno-Precipitations (More)
  • Immuno-Staining Cells/ECM
  • In-Situ Hybridizatioin Slide Preparation
  • Iodination of VWF or CMP
  • IPTG Stock Solution
  • Lipofection (more)
  • Lipofection
  • Lysozume Stock Solution
  • Metabolic Labeling
  • Mung Bean Nuclease
  • Native Gel Electrophoresis
  • Native Reduction-Alkylation of Link Protein/CMP
  • Neutralizing Buffer for Southerns
  • Nitrous Acid
  • NZCYM Medium
  • PBS 10x With or Without Tween-20
  • PEG/DMSO Competent E.Coli
  • Phenol/Chloroform Extractions
  • Poly-A RNA Selection with Oligo-DT cellulose
  • Precipitation of DNA
  • Protease Inhibitor Stocks
  • Protein Gels and Westerns
  • Proteoglycan Agarose Gels
  • RNase, DNase-Free
  • Safranin-O Stain Paraffin-embedded Sections
  • Salmon Sperm DNA
  • Sequencing dsDNA
  • Sequenase Protocol
  • Sequencing Quick-Denature protocol
  • Siliconizing Glassware
  • Silver-Staining PAGE gels
  • SM Medium
  • SOB Medium
  • Sodium Sulfate Precipitation of IgG
  • TBS, 10x
  • TCA Precipitation of Proteins
  • Vimentin-GFP Fixation with Phalloidin/Antibody Counterstain
  • Vimentin Determination of Soluble vs Filamentous Fraction
  • Virus Production
  • Viral Transduction of Chondrocytes
  • Zeller's Solution for Blocking Westerns
    • Logbooks and forms etc.
  • Human Cell Logbook(in pdf)