Alk Phos Assay 96-well kinetic
Dominik Haudenschild From Reddi Lab April 16, 2001
Process Sample
use standard Reddi Lab protocol
Weigh
Homogenize in correct buffer
Do assay
50µl Sodium Barbituate buffer
25µl Sigma 104 buffer
25µl Extract
Kinetic assay read 405nm
Multiply by correction factor
1mOD/min corresponds to XXX Reddi Units
Analyze