Dominik Haudenschild, 10-28-94

To 900ml Milli-Q water add:

NaCl 5g

NZ-Amine 10g (NZ)

Casamino Acids 1g (C)

Bacto-Yeast Extract 5g (Y)

-Stir until dissolved

-Adjust pH to 7.0 with 1N or 5N NaOH

-for Liquid Broth add NO Agarose or Agar

OR -for Top Agarose add 3.75g Agarose per 500ml

OR -for Plates add 7.50g Agar per 500ml

-Bring volume to 1000ml with Milli-Q water

-Autoclave for on Liquid Cycle

-After autoclaving, swirl to thoroughly mix agar

-Cool to 60'C in water bath, add necessary antibiotics

-Pour into 40-50 100mm plates or 16-24 150mm plates

-Use leveling table, avoid bubbles

-Same as NCZYM, but leave out Casamino Acids

-Same as NZCYM but leave out Casamino acids and

leave out Bacto Yeast Extract