Chondrogenic
Media
2009, Dominik Haudenschild, UC Davis
Chondrogenic Base Media
permissive of chondrogenesis
á DMEM with 4.5g/L Glucose
o 4mM L-Glutamine
o 100µg/ml Na-Pyruvate
á 10mM HEPES pH 7.5
á 40µg/ml L- Proline
á 50µg/ml L-ascorbic acid-2-phosphate
á 100nM Dexamethasone
á 1% ITS+
á
{+10mM
CaCl2 in Alginate}
Chondrogenic Media I
promotes chondrogenesis
á Base chondrogenic Media
o + 10ng/ml TGF-§1
Chondrogenic Media II
strongly promotes chondrogenesis
á Base Chondrogenic Media
o + 10ng/ml TGF-§1
o + 100ng/ml BMP-7
500ml bottle (Invitrogen 11995, already contains glu-pyr)
add 10ml of 200mM glutamine
(Invitrogen 25030-081)
add 5ml of 100mM pyruvate (GIBCO 11360, VMCS GB132)
10ml of 0.5M HEPES (VMBMS , Teknova H1575)
add 1ml of 20mg/ml proline (Sigma P-5607, in H20, FS)
add 1ml of 25mg/ml ascorbic acid (Sigma A8960, in H20, FS)
add 1ml of 1:150 dilution of 4mg/ml dexamethasone
add 5.5ml of ITS+ (Clontech 354352)